5IGH

Macrolide 2'-phosphotransferase type I

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-29
Detector	RAYONIX MX-225
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	84.100, 84.100, 98.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.790 - 1.550
R-factor	0.151
Rwork	0.151
R-free	0.16400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.277
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (DEV_1034)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.790	1.590
High resolution limit [Å]	1.550	1.550
Rmerge	0.043	0.468
Number of reflections	55320
<I/σ(I)>	23.46	2.73
Completeness [%]	94.1	69.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	295	0.1 M bicine, 2.2 M ammonium sulfate, 5% glycerol, 3% PEG 400