5IG9
Crystal structure of macrocyclase MdnC bound with precursor peptide MdnA from Microcystis aeruginosa MRC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 41 |
| Unit cell lengths | 132.560, 132.560, 198.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.680 - 2.665 |
| R-factor | 0.2292 |
| Rwork | 0.219 |
| R-free | 0.26050 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.597 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.560 | 2.710 |
| High resolution limit [Å] | 2.665 | 2.660 |
| Rmerge | 0.114 | 0.670 |
| Number of reflections | 96926 | |
| <I/σ(I)> | 17.1 | 3.8 |
| Completeness [%] | 99.9 | 92.8 |
| Redundancy | 11.9 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 297 | 250 mM ammonium sulfate, 28% PEG 3,350, 8% dioxane and 100mM bis-Tris, pH 5.5 |
