5IG7
Crystal structure of anti-gliadin 1002-1E01 Fab fragment in complex of peptide PLQPQQPFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.900, 112.670, 104.950 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 55.869 - 1.970 |
| R-factor | 0.1919 |
| Rwork | 0.190 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ihz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.928 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.870 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 127251 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M ammonium sulfate, 0.1 M Tris, pH 7.5, 20 % w/v PEG 5000 MME |
