5IG1
Crystal structure of S. rosetta CaMKII kinase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 |
Unit cell lengths | 100.031, 100.031, 123.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.580 - 2.900 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.599 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.580 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.103 | 0.700 |
Number of reflections | 27096 | |
<I/σ(I)> | 8.4 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 295 | 1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic pH 6.9 |