5IEU
Crystal Structure of Mycobacterium Tuberculosis ATP-independent Proteasome Activator Tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.040, 63.540, 129.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.810 - 2.800 |
| R-factor | 0.25913 |
| Rwork | 0.258 |
| R-free | 0.28211 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.076 | 0.392 |
| Number of reflections | 5953 | |
| <I/σ(I)> | 6.1 | 4.8 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 0.1 M Tris-HCl and 2 M ammonium sulfate |
