5IET
Crystal Structure of Mycobacterium Tuberculosis ATP-independent Proteasome activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 100.520, 100.520, 229.191 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.990 - 2.880 |
| R-factor | 0.19716 |
| Rwork | 0.196 |
| R-free | 0.21489 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.000 |
| High resolution limit [Å] | 2.880 |
| Number of reflections | 16296 |
| <I/σ(I)> | 15.7 |
| Completeness [%] | 99.9 |
| Redundancy | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 293 | 0.2 M ammonium acetate, 0.1 M sodium citrate 29% 2-methyl-2,4-pentanediol |
