5IEH
Structure of HLA-B*40:02 in complex with the phosphorylated endogenous peptide REF(p)SKEPEL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00556 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.044, 82.249, 110.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.863 - 1.500 |
R-factor | 0.1619 |
Rwork | 0.161 |
R-free | 0.18330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.012 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10) |
Data quality characteristics
Overall | |
Number of reflections | 128337 |
<I/σ(I)> | 22.9 |
Completeness [%] | 99.9 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 150 mM Tris 8.0, 20% PEG5K, 8% ethylene glycol |