5IDL
Crystal structure of the germline-targeting HIV-1 gp120 engineered outer domain, eOD-GT8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.330, 53.060, 77.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.422 - 2.900 |
R-factor | 0.2575 |
Rwork | 0.253 |
R-free | 0.29710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jpj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.455 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.920 | |
High resolution limit [Å] | 2.840 | 12.710 | 2.840 |
Rmerge | 0.131 | 0.033 | 0.779 |
Number of reflections | 3333 | ||
<I/σ(I)> | 6.93 | 24.59 | 1.4 |
Completeness [%] | 99.4 | 95.7 | 98.9 |
Redundancy | 5.8 | ||
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2% (v/v) PEG 400, 2M ammonium sulfate, 0.1M HEPES pH 7.5 |