5IDJ
Bifunctional histidine kinase CckA (domains DHp-CA) in complex with ADP/Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 164.856, 164.856, 48.001 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.590 - 3.020 |
R-factor | 0.2416 |
Rwork | 0.239 |
R-free | 0.28860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5idm |
RMSD bond length | 0.011 |
RMSD bond angle | 1.645 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.598 | 3.170 |
High resolution limit [Å] | 3.010 | 3.010 |
Rmerge | 0.327 | |
Number of reflections | 7413 | 7413 |
<I/σ(I)> | 15 | 2.2 |
Completeness [%] | 91.4 | 51.2 |
Redundancy | 11.3 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG 550 MME, 30% PEG 2000 MME, Morpheus buffer 1 pH 6.5 and Morpheus carboxylic acids |