5IDB
Crystal structure of CGL1 from Crassostrea gigas, mannose-bound form (CGL1/MAN2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-01-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.320, 57.930, 80.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.390 - 1.000 |
| R-factor | 0.12338 |
| Rwork | 0.122 |
| R-free | 0.14400 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 23.500 |
| High resolution limit [Å] | 1.000 |
| Number of reflections | 131088 |
| <I/σ(I)> | 16.4 |
| Completeness [%] | 89.0 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 293 | 30% PEG 3000, 100mM CHES |
