5IDA
Crystal structure of CGL1 from Crassostrea gigas, mannose-bound form (CGL1/MAN)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-11-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.723, 58.778, 108.460 |
Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
Resolution | 17.870 - 1.100 |
R-factor | 0.16705 |
Rwork | 0.164 |
R-free | 0.22207 |
RMSD bond length | 0.028 |
RMSD bond angle | 2.447 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.100 |
Number of reflections | 160510 |
<I/σ(I)> | 17 |
Completeness [%] | 79.6 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2 M magnesium acetate, 0.1 M sodium cacodylate, 20% PEG8000 |