5ID6

Structure of Cpf1/RNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-10
Detector	CCD pilatus CBF
Wavelength(s)	0.97776
Spacegroup name	C 1 2 1
Unit cell lengths	168.352, 83.011, 124.086
Unit cell angles	90.00, 106.72, 90.00

Refinement procedure

Resolution	41.506 - 2.382
R-factor	0.2052
Rwork	0.202
R-free	0.26080
RMSD bond length	0.009
RMSD bond angle	1.388
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.510	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	64262
<I/σ(I)>	20.67
Completeness [%]	99.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.16 M Magnesium acetate, 18%(w/v) Polyethylene glycol 3350