5ID0

Cetuximab Fab in complex with aminoheptanoic acid-linked meditope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.060, 82.710, 212.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.740 - 2.480
R-factor	0.163
Rwork	0.160
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.740	2.540
High resolution limit [Å]	2.480	2.480
Rmerge	0.048	0.186
Number of reflections	40610
<I/σ(I)>	28.9	7.15
Completeness [%]	98.7	89.6
Redundancy	5.85	4.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M CITRIC ACID, 0.1 M SODIUM HYDROGEN PHOSPHATE, 0.4 M POTASSIUM HYDROGEN PHOSPHATE, 1.6 M SODIUM DIHYDROGEN PHOSPHATE, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K