5ICH
Crystal structure of Enterococcus faecalis lipoate-protein ligase A (lplA-2) in complex with 8BO-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97905 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.917, 69.383, 97.664 |
Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
Resolution | 21.500 - 1.850 |
R-factor | 0.1712 |
Rwork | 0.169 |
R-free | 0.20520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iby |
RMSD bond length | 0.010 |
RMSD bond angle | 1.186 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP (11.0.05) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.037 | 0.026 | 0.126 |
Rmeas | 0.041 | 0.030 | 0.145 |
Rpim | 0.018 | 0.013 | 0.070 |
Total number of observations | 282553 | ||
Number of reflections | 58160 | ||
<I/σ(I)> | 23.7 | ||
Completeness [%] | 99.8 | 98.2 | 100 |
Redundancy | 4.9 | 4.7 | 4.1 |
CC(1/2) | 0.999 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 289 | 1.5 UL PROTEIN + 1.5 UL BUFFER (27% PEG3350, 0.1 M SODIUM CACODYLATE, 0.2 M SODIUM CHLORIDE, PH 5.25) |