5ICA
Structure of the CTD complex of UTP12, Utp13, Utp1 and Utp21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 121.695, 121.695, 167.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.382 - 3.507 |
R-factor | 0.247 |
Rwork | 0.244 |
R-free | 0.27500 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.069 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.172 | |
Number of reflections | 34729 | |
<I/σ(I)> | 14.6 | 1.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20.7 | 20.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES Sodium, 45% w/v Poly(acrylic acid sodium salt) 5100, 0.02M Magnesium Chloride, 0.01M Spermidine |