5IC9
Structure of the CTD complex of Utp12 and Utp13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 160.311, 160.311, 134.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.900 - 3.700 |
| R-factor | 0.2773 |
| Rwork | 0.275 |
| R-free | 0.29940 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8.4_1496) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.760 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.168 | |
| Number of reflections | 20816 | |
| <I/σ(I)> | 19.2 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 24.3 | 24.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris-HCl, 50% Pentaerythritol, 0.35M Ammonium Sulfate, 0.2M Sodium Thiocyanate |
