5IBZ
Crystal structure of a novel cyclase (pfam04199).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.508, 120.618, 148.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.814 - 1.611 |
| R-factor | 0.1342 |
| Rwork | 0.133 |
| R-free | 0.16140 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.814 | 1.640 |
| High resolution limit [Å] | 1.611 | 1.611 |
| Rmerge | 0.083 | 0.484 |
| Number of reflections | 150117 | |
| <I/σ(I)> | 17 | 2.8 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 5.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2KMME 30%, Tris pH 8 0.1M, NH4Acet 0.2M |
