5IBV
Crystal Structure of Human Astrovirus capsid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | PIXEL |
Collection date | 2014-11-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.983, 59.236, 56.197 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.150 |
R-factor | 0.2132 |
Rwork | 0.209 |
R-free | 0.24580 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.207 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.066 | 0.507 |
Number of reflections | 18278 | |
<I/σ(I)> | 17.9 | 2 |
Completeness [%] | 99.8 | 97.8 |
Redundancy | 4 | 3.2 |
CC(1/2) | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | VAPOR DIFFUSION, HANGING DROP, 0.2 M ammoium phosphate, 22% PEG 4000 |