5IBL
Human antibody 6639 in complex with influenza hemagglutinin H1 X-181
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 173.15 |
Detector technology | CCD |
Collection date | 2014-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979200 |
Spacegroup name | P 1 |
Unit cell lengths | 54.130, 104.720, 116.660 |
Unit cell angles | 101.95, 95.33, 98.36 |
Refinement procedure
Resolution | 44.810 - 3.390 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ibu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.810 | 3.590 |
High resolution limit [Å] | 3.390 | 3.390 |
Rmerge | 0.178 | 1.730 |
Number of reflections | 32602 | |
<I/σ(I)> | 4.3 | 0.48 |
Completeness [%] | 95.5 | 94 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 12% (w/v) PEG 8000, 10% (w/v) MPD and 25 mM KH2PO4 |