5IB7
Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.300, 448.800, 620.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.082 - 2.990 |
| R-factor | 0.2016 |
| Rwork | 0.202 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e7k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.581 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 147.082 | 3.070 | |
| High resolution limit [Å] | 2.990 | 13.370 | 2.990 |
| Rmerge | 0.344 | 0.061 | 4.673 |
| Number of reflections | 1162817 | ||
| <I/σ(I)> | 14.73 | 80.5 | 1 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 69.52 | ||
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |
