5IAU
A C69-family cysteine dipeptidase from Lactobacillus farciminis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-17 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.170, 182.281, 97.967 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 38.877 - 1.781 |
| R-factor | 0.1537 |
| Rwork | 0.152 |
| R-free | 0.18130 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.877 | 1.810 |
| High resolution limit [Å] | 1.781 | 1.780 |
| Rmerge | 0.058 | 0.388 |
| Number of reflections | 254496 | |
| <I/σ(I)> | 41.6 | 5.41 |
| Completeness [%] | 99.2 | 95.9 |
| Redundancy | 6.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.8 M 1,6-hexanediol, 10 mM CoCl2, 100 mM acetate (pH 4.8) |
