5IA7
Crystal structure of Ubiquitin fold modifier 1 (Ufm1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.282, 56.730, 64.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.580 - 2.000 |
| R-factor | 0.19968 |
| Rwork | 0.198 |
| R-free | 0.22961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iaa |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.749 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.450 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.142 | |
| Number of reflections | 11636 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M Ammonium acetate, 0.1M Sodium citrate tribasic dihydrate and 30% PEG 4000 |
