5I9J
Structure of the cholesterol and lutein-binding domain of human STARD3 at 1.74A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11583 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.390, 83.390, 82.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.180 - 1.740 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.19190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1em2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.161 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev-2276) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.180 | 1.790 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.046 | 0.810 |
Number of reflections | 34160 | |
<I/σ(I)> | 25.8 | 2.4 |
Completeness [%] | 99.5 | 98.6 |
Redundancy | 8.8 | 6.8 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 295 | 0.85 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1 M CHES, 0.01 M dithiothreitol. Cryostabilizer: 18% ethylene glycol, 5% ethanol in addition to 0.8M sodium/potassium tartrate, 0.1 M CHES pH 9.1, 0.2 M lithium sulfate, 0.01 M dithiothreitol. Crystals were frozen in propane and stored under liquid nitrogen |