5I9H

Crystal structure of designed pentatricopeptide repeat protein dPPR-U8G2 in complex with its target RNA U8G2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9784
Spacegroup name	P 21 21 21
Unit cell lengths	52.063, 85.098, 95.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.699 - 2.504
R-factor	0.2268
Rwork	0.223
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pjr
RMSD bond length	0.007
RMSD bond angle	0.917
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	15179
<I/σ(I)>	19.8
Completeness [%]	99.8
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, Bis-Tris propane, Magnesium chloride, Ethyl Acetate, D-(+)-glucose monohydrate