5I9D
Crystal structure of designed pentatricopeptide repeat protein dPPR-U8A2 in complex with its target RNA U8A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9784 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.546, 84.904, 95.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.082 - 2.596 |
| R-factor | 0.2683 |
| Rwork | 0.267 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pjr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.789 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.700 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 13826 |
| <I/σ(I)> | 16.7 |
| Completeness [%] | 99.8 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Ethyl Acetate,D-(+)-glucose monohydrate |
