5I92
Crystal structure of Glutamate-1-semialdehyde 2,1- aminomutase (GSA) from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.660, 121.440, 142.740 |
Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
Resolution | 47.503 - 1.750 |
R-factor | 0.153 |
Rwork | 0.153 |
R-free | 0.17880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | PHENIX ((dev_2313)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.520 | 1.800 | |
High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
Rmerge | 0.056 | 0.021 | 0.482 |
Rmeas | 0.064 | 0.024 | 0.550 |
Total number of observations | 1022137 | ||
Number of reflections | 241258 | 2744 | 17795 |
<I/σ(I)> | 18.2 | 50.11 | 3.33 |
Completeness [%] | 99.9 | 98.2 | 99.9 |
CC(1/2) | 0.999 | 0.999 | 0.864 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | MCSG-1 (D9): 25% PEG-3350, 0.2M NACL, 0.1M TRIS, PH=8.5, CRYO: 20% EG |