5I90
Crystal Structure of PvdN from Pseudomonas Aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-15 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.521, 98.996, 63.046 |
Unit cell angles | 90.00, 102.32, 90.00 |
Refinement procedure
Resolution | 29.407 - 1.219 |
R-factor | 0.1405 |
Rwork | 0.140 |
R-free | 0.15830 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.407 | 1.263 |
High resolution limit [Å] | 1.219 | 1.219 |
Number of reflections | 221938 | |
<I/σ(I)> | 22.92 | |
Completeness [%] | 99.9 | 98.99 |
Redundancy | 4.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 287 | 10-14% PEG 8000, 100-200 mM Glycine, 50 mM MES (pH 6.0) |