5I8O

HMM5 Fab in complex with disaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-14
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	63.447, 63.587, 108.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.913 - 1.800
R-factor	0.1663
Rwork	0.164
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.913		1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.170	0.060	0.982
Number of reflections	41574
<I/σ(I)>	9.05	25.05	1.72
Completeness [%]	100.0	99.8	100
Redundancy	6.594
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	15-25 mM LiSO4, 15-26.5% (w/v) PEG 3350, 0.1M Tris or HEPES pH 7.0-8.5, 18 mM Fuca1-3GlcNAca1-OMe