5I8J
Crystal structure of Dmd from phage RB69
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.910, 53.933, 75.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.914 - 1.750 |
R-factor | 0.1934 |
Rwork | 0.192 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4I8R |
RMSD bond length | 0.006 |
RMSD bond angle | 0.964 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.084 | 0.333 |
Number of reflections | 13857 | |
<I/σ(I)> | 53.76 | 8.81 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 13.7 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES 25% PEG3350 |