5I8E
Crystal Structure of Broadly Neutralizing HIV-1 Fusion Peptide-Targeting Antibody VRC34.01 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 4 |
Unit cell lengths | 114.606, 114.606, 174.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.314 - 2.655 |
R-factor | 0.1891 |
Rwork | 0.186 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jpi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.532 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.740 |
High resolution limit [Å] | 2.650 | 5.710 | 2.650 |
Rmerge | 0.123 | 0.084 | 0.807 |
Rmeas | 0.144 | ||
Rpim | 0.072 | ||
Total number of observations | 100033 | ||
Number of reflections | 30065 | ||
<I/σ(I)> | 12.9 | ||
Completeness [%] | 92.9 | 88.9 | 95.1 |
Redundancy | 3.3 | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Zinc Acetate, 0.1M Sodium Cacodylate pH6.5 and 15% PEG 8000 |