5I8D
Crystal Structure of Mouse Cadherin-23 EC19-21 S2064P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.722, 67.754, 112.782 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 111.360 - 2.690 |
| R-factor | 0.22052 |
| Rwork | 0.219 |
| R-free | 0.25273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Cadherin-8 (2A62) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.446 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.360 | 2.750 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.058 | 0.454 |
| Number of reflections | 15826 | |
| <I/σ(I)> | 16.38 | 2 |
| Completeness [%] | 98.4 | 94.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 277 | 0.025M Calcium Acetate, 0.1 M Sodium Cacodylate, 25% MPD |
