5I87
Crystal structure of BT-CD domains of human acetyl-CoA carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 267.270, 267.270, 210.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.518 - 3.700 |
R-factor | 0.3485 |
Rwork | 0.347 |
R-free | 0.38070 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.832 |
Data reduction software | XDS (VERSION March 1, 2015) |
Data scaling software | XSCALE (VERSION March 1, 2015) |
Phasing software | SHELXCD (2014/2) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.518 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.075 | 4.010 |
Number of reflections | 77629 | |
<I/σ(I)> | 21.24 | 1.07 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 13.7 | 13.7 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292.15 | MES, tri-potassium citrate, PEG10000 |