5I7K
Crystal Structure of Human SPLUNC1 Dolphin Mutant D1 (G58A, S61A, G62E, G63D, G66D, I67T)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.027, 204.927, 118.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 2.552 |
R-factor | 0.2184 |
Rwork | 0.212 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kgh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.504 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 2.644 |
High resolution limit [Å] | 2.552 | 2.552 |
Rmerge | 0.057 | 0.657 |
Number of reflections | 19300 | |
<I/σ(I)> | 13.8 | 2.11 |
Completeness [%] | 98.7 | 89.42 |
Redundancy | 3.5 | 3.2 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 310 | 6M Ammonium Nitrate, and 0.1M Tris HCL pH 8.5 |