5I7J
Crystal Structure of Human SPLUNC1 Disulfide Mutant M3 (I76C, V214C)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-BM-B |
Synchrotron site | APS |
Beamline | 23-BM-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.778, 206.163, 117.649 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.283 - 2.544 |
R-factor | 0.2062 |
Rwork | 0.200 |
R-free | 0.26770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kgh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.484 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.283 | 2.634 |
High resolution limit [Å] | 2.544 | 2.544 |
Rmerge | 0.055 | 0.706 |
Number of reflections | 19395 | |
<I/σ(I)> | 14.54 | |
Completeness [%] | 98.7 | 94.45 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 310 | 6M Ammonium Nitrate, 0.1M Tris-HCL, pH 8.5 |