5I7G
Metal free Glucose Isomerase collected at room temperature using the HC1b humidity controller
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 295 |
| Detector technology | PIXEL |
| Collection date | 2014-10-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.815, 99.698, 102.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.378 - 1.210 |
| R-factor | 0.1383 |
| Rwork | 0.137 |
| R-free | 0.16170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.606 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PH |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.280 | 1.240 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.121 | 1.680 |
| Number of reflections | 145645 | |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10 mM Hepes pH 7.5, 1.6 M Ammonium Sulphate |
