5I6S

Crystal structure of an endoglucanase from Penicillium verruculosum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	CCD
Collection date	2014-10-20
Detector	Bruker Platinum 135
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	83.819, 83.819, 89.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.222 - 2.100
R-factor	0.2203
Rwork	0.218
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gzj
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.222	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.071	0.537
Number of reflections	19130
<I/σ(I)>	10.96
Completeness [%]	99.9
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	20 % w/v Polyethylene glycol 6,000 50 mM BIS-TRIS propane; pH 7.0 7 % v/v 2-Methyl-2,4-pentanediol, 5 % v/v 2-Methyl-2-propanol, 6% ???PEG 5000