5I6J
Crystal Structure of SRGAP2 F-BARx
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.170, 29.900, 94.730 |
| Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
| Resolution | 101.530 - 2.700 |
| R-factor | 0.20661 |
| Rwork | 0.203 |
| R-free | 0.26275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.678 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.360 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 16087 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 98.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 293.15 | F-BARx-WT was eluted from the anion exchange column (MonoQ-GL10/100, GE Healthcare) in buffer containing 5% Glycerol, 25 mM Tris buffer pH=7.4, 5 mM ?-mercaptoethanol, and ~0.16 M NaCl at the elution peak and concentrated to 18 mg/ml. F-BARx-WT crystals grew in 0.2 M ammonium citrate tribasic pH=7.25, 35% PEG1500. |
