5I6I
Crystal structure of a dBCCP-variant of Chaetomium thermophilum acetyl-CoA carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.99986 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 462.200, 462.200, 204.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.950 - 8.400 |
R-factor | 0.2981 |
Rwork | 0.297 |
R-free | 0.32360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i6f 5i6e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 |
Data reduction software | XDS (VERSION March 1, 2015) |
Data scaling software | XSCALE (VERSION March 1, 2015) |
Phasing software | PHASER (2.5.7) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.950 | 8.620 |
High resolution limit [Å] | 8.400 | 8.400 |
Rmerge | 0.294 | 3.820 |
Number of reflections | 12113 | |
<I/σ(I)> | 9.05 | 0.9 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 18.5 | 18.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | Morpheus buffer system 3, Morpheus ethylene glycol mix, PEG4000, Glycerol |