5I6H
Crystal structure of CD-CT domains of Chaetomium thermophilum acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 295.020, 295.020, 189.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.850 - 7.200 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6f 5i6e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS (VERSION March 1, 2015) |
| Data scaling software | XSCALE (VERSION March 1, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.850 | 7.390 |
| High resolution limit [Å] | 7.200 | 7.200 |
| Rmerge | 0.056 | 3.030 |
| Number of reflections | 14050 | |
| <I/σ(I)> | 18.95 | 0.92 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.9 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292.15 | Bicine, PEG8000 |
