5I6G
Crystal structure of C-terminal variant 2 of Chaetomium thermophilum acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.170, 153.450, 249.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.100 - 4.500 |
| R-factor | 0.2358 |
| Rwork | 0.236 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS (VERSION March 1, 2015) |
| Data scaling software | XSCALE (VERSION March 1, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.670 | 4.820 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.274 | 2.260 |
| Number of reflections | 23225 | |
| <I/σ(I)> | 6.35 | 1 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 6.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | Hepes, Tacsimate, PEGMME5000 |
