5I6E
Crystal structure of the central domain of yeast acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.991880 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 110.860, 110.860, 131.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 3.000 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.24370 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS (VERSION March 1, 2015) |
| Data scaling software | XSCALE (VERSION March 1, 2015) |
| Phasing software | SHELXCD (2014/2) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.370 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.182 | 3.900 |
| Number of reflections | 16929 | |
| <I/σ(I)> | 24.68 | 1.46 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 39.1 | 39.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292.15 | BisTrisPropane, di-sodium malonate, PEG3350, trimethylamine |
