5I66

X-ray structure of the Fab fragment of 8B6, a murine monoclonal antibody specific for the human serotonin transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	81.560, 81.560, 142.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.672 - 1.624
R-factor	0.1792
Rwork	0.178
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lqf
RMSD bond length	0.012
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	57.672
High resolution limit [Å]	1.620
Rmerge	0.052
Number of reflections	117803
<I/σ(I)>	18.9
Completeness [%]	97.7
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM HEPES pH 7.5, 300 mM NH4H2PO4 and 22.5% pure PEG (0.3-0.8 kDa)