5I62

Crystal structure of the insertion loop deletion mutant of the RNA-dependent RNA polymerase of a human picorbirnavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	CCD
Collection date	2012-03-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.12719
Spacegroup name	P 41 21 2
Unit cell lengths	77.534, 77.534, 183.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.721 - 2.001
R-factor	0.1733
Rwork	0.172
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Crystal structure of the RNA-dependent RNA polymerase of a human picorbirnavirus
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.090	0.045	0.260
Total number of observations	505906
Number of reflections	38794
<I/σ(I)>	19.6
Completeness [%]	99.9	98.8	100
Redundancy	13	12.6	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	200 mM sodium acetate, 100 mM sodium cacodylate pH 6.5, and 35% PEG 8000