5I62
Crystal structure of the insertion loop deletion mutant of the RNA-dependent RNA polymerase of a human picorbirnavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-03-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12719 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.534, 77.534, 183.858 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.721 - 2.001 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.20120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Crystal structure of the RNA-dependent RNA polymerase of a human picorbirnavirus |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.090 | 0.045 | 0.260 |
Total number of observations | 505906 | ||
Number of reflections | 38794 | ||
<I/σ(I)> | 19.6 | ||
Completeness [%] | 99.9 | 98.8 | 100 |
Redundancy | 13 | 12.6 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 200 mM sodium acetate, 100 mM sodium cacodylate pH 6.5, and 35% PEG 8000 |