5I5L

The photosensory module (PAS-GAF-PHY) of the bacterial phytochrome Agp1 (AtBphP1) in the Pr form, chromophore modelled with an endocyclic double bond in pyrrole ring A

Replaces:  4WKK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	I 4 2 2
Unit cell lengths	173.019, 173.019, 78.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.550 - 2.700
R-factor	0.2187
Rwork	0.216
R-free	0.27901
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hsq
RMSD bond length	0.009
RMSD bond angle	1.447
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.061	0.640
Number of reflections	16105
<I/σ(I)>	20	3.3
Completeness [%]	96.7	87.7
Redundancy	7.3	6.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	16% PEG3350, 0.2 M CaCl2, 0.1 M Tris/HCl