5I4F
scFv 2D10 complexed with alpha 1,6 mannobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 173.15 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.093, 81.093, 74.388 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.114 - 1.549 |
R-factor | 0.1697 |
Rwork | 0.167 |
R-free | 0.19310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h0g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.593 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.549 | 1.549 |
Rmerge | 0.046 | 0.506 |
Number of reflections | 41534 | |
<I/σ(I)> | 39.67 | 2.9 |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | MES 6.5, Magnesium sulphate (1.5 M), potassium sodium tartrate tetrahydrate (0.1M). |