5I4A

X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587,0.97964,0.97197
Spacegroup name	P 1
Unit cell lengths	50.883, 75.420, 102.215
Unit cell angles	110.87, 90.55, 90.09

Refinement procedure

Resolution	48.327 - 1.949
R-factor	0.2161
Rwork	0.216
R-free	0.23870
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.578
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.330	1.980
High resolution limit [Å]	1.949	1.949
Rmerge	0.082	1.136
Number of reflections	100295
<I/σ(I)>	10.6	1.2
Completeness [%]	96.7	82.1
Redundancy	3.9	3.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	27% PEG 2,000, 0.1M HEPES