5I45
1.35 Angstrom Crystal Structure of C-terminal Domain of Glycosyl Transferase Group 1 Family Protein (LpcC) from Francisella tularensis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.484, 58.962, 41.646 |
Unit cell angles | 90.00, 117.65, 90.00 |
Refinement procedure
Resolution | 29.550 - 1.350 |
R-factor | 0.16355 |
Rwork | 0.162 |
R-free | 0.20303 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB-3C4Q |
RMSD bond length | 0.011 |
RMSD bond angle | 1.560 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.073 | 0.660 |
Number of reflections | 36249 | |
<I/σ(I)> | 35.5 | 2.6 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 4.9 | 4.9 |
CC(1/2) | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292 | Protein: 10.4 mg/ml, 0.01M Tris-HCL (pH 8.3); Screen: PACT (D6), 0.1M MMT buffer (pH 9.0), 25% (w/v) PEG 1500. |