5I44
Structure of RacA-DNA complex; P21 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.600, 68.500, 117.400 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.621 |
| Rwork | 0.239 |
| R-free | 0.26880 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 500.000 |
| High resolution limit [Å] | 2.620 |
| Number of reflections | 49675 |
| <I/σ(I)> | 15.5 |
| Completeness [%] | 98.6 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3000, 0.1 M Tris 8.0, lithium sulphate |
