5I41

Structure of the apo RacA DNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-04
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.780, 34.536, 42.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.149 - 1.800
R-factor	0.1832
Rwork	0.180
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.550
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.150
High resolution limit [Å]	1.800
Number of reflections	4854
<I/σ(I)>	12.5
Completeness [%]	98.1
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20 mM Tris pH 7.5, 100 mM NaCl