5I34

Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	55.807, 100.774, 163.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.118 - 1.530
R-factor	0.1347
Rwork	0.134
R-free	0.18560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i33
RMSD bond length	0.009
RMSD bond angle	1.184
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.120	1.580
High resolution limit [Å]	1.530	1.530
Rmerge	0.077	0.680
Number of reflections	140564
<I/σ(I)>	16.6
Completeness [%]	99.9	99.4
Redundancy	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M ammonium citrate tribasic 18% PEG3350